The effects of pH and substrate concentration on enzyme activity ..

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The effect of enzyme concentration on enzyme activity essay.



Concentration Of Catalase Enzyme

Experiment Attempts

Catalase

Water

1st

2nd

3rd

(100%) 5ml

(0%) 0ml

ml

ml

ml

(80%) 4ml

(20%) 1 ml

ml

ml

ml

(60%) 3 ml

(40%) 2ml

ml

ml

ml

(40%) 2ml

(60%) 3ml

ml

ml

ml

(20%) 1 ml

(80%) 4ml

ml

ml

ml

Conclusion

From the results I had gathered after doing this experiment I found
that when I increase the concentration of the Catalase Enzyme (by
adding more liquidised potato parts to water parts) the rate of the
reaction is greater and this affects the enzyme activity.

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The Effect of pH on Enzyme Activity :: Papers

(H O ) Prediction: I predict that with the higher concentration of enzyme, the likelihood of it breaking down molecules will be greater because there will be more enzymes to work at the substrate and the chances of it colliding will be higher making the activity time quicker....

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Pancreatic lipase (PL),one of the exocrine enzymes of pancreatic juice, catalyzes the hydrolysis of emulsified esters of glycerol and long chain fatty acids. The substrate is not a single molecule but a nonaqueous phase of aggregated lipid (Brockerhoff and Jensen 1974). The operative substrate characteristic is aggregates of ester molecules, micelles or monomolecular film, interfacing an aqueous medium. Enzyme activity is directly related to the concentration of substrate molecules on the interface (Esposito et al 1973; Lagocki et al 1973. PL attacks the primary ester groups most readily. Monoglycerides are poor substrates (it is the 2-monoglycerides that are absorbed through the intestinal wall and reformed into lymph chlyomicrons). Pancreatic lipases have been thoughly reviewed by Brockerhoff and Jensen (1974), and Desnuell (1972). Liberman and Ollis (1975) have reported on lipase immobilized on stainless steel and polyacrylamide beads. Using a fluidized bed recycle reactor it is indicated that enzyme-substrate affinity is not altered.

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